Free bioinformatics tools that run in your browser
Every tool on this page is a real bioinformatics program — samtools, bcftools, bedtools, seqtk, fastp, minimap2 — compiled to WebAssembly and executed inside your browser tab. There is nothing to install, no container to configure, no queue to wait in and no file to upload. The commands produce the same output you would get from a local install, which means you can learn or check a result at a bench, in a meeting, or on a machine you do not administer.
That last point is more important than it sounds. Genomic data, clinical results and unpublished manuscripts are frequently confidential or embargoed, and sending them to a third-party web server is a poor default. Because the compute happens on your device, you can work on sensitive material without transferring a single byte. The trade-off is that large files are bounded by your device memory, so whole-genome work still belongs on a proper machine.
Where to start
If you are new to sequence analysis, work in the order the data arrives rather than the order that sounds most interesting:
- Assess the reads. Start with FASTQ Quality Control to see per-base quality, detect adapters and decide on trimming. Skipping this step is the most common cause of a disappointing alignment rate later.
- Check the reference. Use FASTA and FASTQ Tools to confirm base composition and GC content of your reference before trusting anything built on it.
- Align. Sequence Alignment maps reads to the reference using the preset for your platform.
- Verify the alignment. SAM/BAM Viewer reads the header and flagstat so you know the mapping actually worked.
- Call or inspect variants. VCF Variant Analyzer examines the call set, and BED Tools handles the interval questions that follow.
Built for people who work with real results
Your data stays yours
Files are read into browser memory and never transmitted. The privacy policy sets out exactly what is and is not collected.
Real programs, real versions
These are the same open-source tools used in published pipelines, pinned to the versions shown on each page, so output is comparable with what a colleague gets locally.
Every page documents itself
Each tool page explains what it does, gives worked command examples and answers the questions people actually ask, so you rarely need a manual to get started.
Verification, not just analysis
Two tools are built specifically for checking results others produced: a sequencing verification report and a plagiarism and AI-content checker.
How It Works
All tools run entirely in your browser using WebAssembly. Your data never leaves your computer - everything is processed locally for maximum privacy and security.
Click on any tool below to get started. Upload your files and run commands just like you would on the command line!
SAM/BAM Viewer
View and analyze alignment files using samtools
FASTA / FASTQ Tools
Sequence processing and FASTA/FASTQ manipulation using Seqtk.
VCF Analyzer
Analyze variants, SNPs, and genomic mutation data using Bcftools.
BED Tools
Perform genome interval arithmetic and overlap analysis.
FASTQ Quality Control
Quality filtering, trimming, and FASTQ preprocessing using Fastp.
Sequence Alignment
Align long-read and short-read genomic sequences using Minimap2.
Genome Ribbon
Interactive visualization for structural variants and genomic rearrangements.
NCBI FASTA Analyzer
Find Mutation & Their Plot, CSV Data & Log File
Academic Plagiarism Checker
Check academic documents for plagiarism, AI content, citations, and scholarly sources.
Sequencing Result Verification
Verify a company-provided FASTA sequence against your expected PCR product with alignment evidence.
Sequence File Converter
Convert and analyze FASTA, FASTQ, FASTQ.gz, AB1, GenBank, and CSV/TSV sequence files in your browser.
BioSim Workbench
Simulate biochemical reaction networks with adaptive RK45/BDF and Gillespie solvers, fit rate constants, and export CSV, SBML and PDF.
Frequently asked questions
Do these tools upload my data to a server?
No. Every tool on this page runs as WebAssembly inside your browser tab. Files are read into browser memory and processed on your own device, so genome data, clinical results and unpublished sequences never leave your machine.
Do I need to install anything?
No. There is nothing to install, no container to configure and no queue to wait in. Open a page, load a file and run a command. This makes the tools usable on a locked-down laptop, a borrowed machine or a tablet.
Are these the same tools used in published pipelines?
Yes. They are the standard open-source programs, including samtools, bcftools, bedtools, seqtk, fastp and minimap2, compiled to WebAssembly and pinned to the versions listed on each page. Output matches what a local install produces.
Is there a file size limit?
There is a practical limit set by your device memory, because files are loaded into browser memory. Files of a few hundred megabytes are usually fine on a modern laptop. For whole-genome data, run the same commands locally.
Can I use these results in a paper or report?
Yes, but verify them. These tools are for analysis and quality control, not validated clinical software, and the output should be independently checked before being used in a formal or clinical context.
Can I use these on a commercial or confidential project?
From a privacy standpoint, yes. Because nothing is transmitted, you can analyse proprietary, embargoed or patient-derived data here. Check the terms of your own institution and agreements as well, and remember that published work should be reproducible regardless of the tool used.
Do the tools work offline?
Once the page has loaded and the WebAssembly binaries are cached, the core processing works without a network connection, which is useful in laboratories with restricted connectivity.
How do I report a bug or request a feature?
Use the contact page. Please include the exact command, the file format and, if you can, a small synthetic example. Bug reports on real clinical or personal data should never include that data.